Abstract
First-order rate constants for the hydrolysis of polyuridylic acid in the absence and presence of various metal ions and their chelates have been determined under neutral and slightly acidic conditions. The hydrolysis has been shown to proceed by cleavage of non-terminal bonds rather than by stepwise release of monomeric nucleotides. Only the hydrolysis of the 3′,5′-phosphodiester bonds is accelerated by metal ions, not their isomerization to 2′,5′-bonds. The catalytically active species has been shown to be the monohydroxo form of the metal aquo ion. The rate accelerations are parallel, but up to 20 times greater than those obtained with uridylyl (2′,5′)uridine. The mechanism of the metal-ion action is discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
