Metagenomics and network analysis elucidating the coordination between fermentative bacteria and microalgae in a novel bacterial-algal coupling reactor (BACR) for mariculture wastewater treatment

Abstract

To achieve the goal of treating mariculture wastewater economically and efficiently, a novel bacterial-algal coupling reactor (BACR) integrating acidogenic fermentation and microalgae cultivation was firstly investigated for mariculture wastewater treatment. Volatile fatty acids (VFAs) generated in the dark chamber migrated into the photo chamber for microalgal utilization, which alleviated the pH drop and feedback inhibition of the acidogenic fermentation. The maximum dry cell weight (DCW) of microalgae was 1.46 g/L, and pollutants such as chemical oxygen demand (COD), ammonium (NH4+-N) and total phosphorus (TP) in the BACR were effectively removed under the mixotrophic culture condition. Furthermore, bacterial community profiles and functional genes in the BACR and single acidogenic fermentation reactor were identified. Compared with the single acidogenic fermentation reactor, most of the fermentative bacteria (e.g., Ruminococcus, Christensenellaceae R-7 group, Exiguobacterium, Pseudomonas and Levilinea) were enriched by the BACR. From the genetic perspective, the abundances of dominant genes (ackA, acs and atoD) associated with acetic, propionic and butyric acid production were greatly enhanced in the BACR. In the fatty acid biosynthesis pathway (ko00061), three kinds of high-abundance acetyl-CoA carboxylase genes and eight kinds of downstream functional genes were up-regulated in the BACR. Finally, based on co-occurrence network analysis, the coordination between fermentative bacteria and microalgae in the BACR was revealed. This study provided a deep insight into the advantage and potential of the BACR in mariculture wastewater treatment.