Metagenomic sequencing in the diagnosis of resistance to antimicrobials

Abstract

The purpose of the article is to analyze the literature on antimicrobial resistance and the metagenomic sequencing method. In total, 32 articles published in the years 2019–2024 and some previously published classic works were analyzed. Antimicrobial resistance (AMR) is one of the most serious threats to public health, animal health and environmental well-being globally, generating high costs. Metagenomic sequencing allows the detection of all genes in a sample, providing a complete picture of AMR. By sequencing it is possible to assess the location and transmissibility of the AMR genes. As surveillance networks and metagenomic sequencing, as one of the rapid diagnostic platforms, are implemented globally, it will be possible to detect and limit infectious outbreaks at a much earlier stage, thus saving lives and substantially reducing costs. In the near future, metagenomic sequencing will no longer be a luxury but a necessity as we engage in the ongoing fight against infectious diseases.