Metabonomic analysis of the blood serum of crack resocialization process

Ljubica Tasic,Pedro Henrique De Barros Pinto,Tássia Brena Barroso Carneiro Da Costa

doi:10.20396/revpibic2620181135

Abstract

Crack-cocaine is a potent form of cocaine abuse and is considered, globally, as one of the most potentially dangerous illicit drugs. In Brazil, it is one of the most commonly used drugs because it is cheap and its effects are very quick. The identification of biomarkers in crack-cocaine users may add-in the search for new treatments for this drug addicts. This project aims a metabonomics study by ¹H NMR on blood serum samples from healthy and crack-cocaine dependent women. ¹H NMR spectral data were analyzed by statistical analysis techniques, such as principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and sparse PLS-DA (sPLS-DA). Subsequently, important biomarkers for crack-cocaine abuse were identified.

Highlights

Crack, a psychoactive drug, is a huge concern for the public health in Brazil, the world greatest consumer of this illicit drug
The project targets to find metabolites that differentiate healthy volunteers from crack users, through the application of 1H NMR spectroscopy of serum samples, to distinguish the samples in three groups: control (HC), user of crack before the treatment (1) and users of crack after treatment (2) and to identify biomarkers associated with crack dependence and treatment
The 1H-NMR spectra were normalized by the sum and mean centered

Summary

Introduction

A psychoactive drug, is a huge concern for the public health in Brazil, the world greatest consumer of this illicit drug. The constant use of crack brings changes in attention tests, verbal fluency, visual memory and executive functions associated directly to neuropsychological deficits. The project targets to find metabolites that differentiate healthy volunteers from crack users, through the application of 1H NMR spectroscopy of serum samples, to distinguish the samples in three groups: control (HC), user of crack before the treatment (1) and users of crack after treatment (2) and to identify biomarkers associated with crack dependence and treatment.

Objectives

Results

Conclusion