Abstract
Metabolomics analysis using LC-HRESIMS of twelve extracts of Spongia irregularis associated actinomycetes for dereplication purposes in addition to evaluation of cytotoxic and antiviral activities of the extracts. In the present study, three actinomycetes belonging to the genera Micromonospora, Streptomyces, and Rhodococcus were recovered from the marine sponge Spongia irregularis. Applying OSMAC approach, each strain was fermented on four different media resulting in twelve extracts. All extracts were subjected to metabolomics analysis using LC-HRESIMS for dereplication purposes. Multivariate data statistical analysis was carried out for the differentiation between extracts. Additionally, the cytotoxic and anti-HCV potentials of extracts were evaluated. Most of extracts showed strong to moderate cytotoxicity effects against HepG-2, CACO-2 and MCF-7 cell lines with general IC50 range of 2.8-8.9µg/mL. Moreover, the extracts of Micromonospora sp. UR44 using ISP2 and OLIGO media and Streptomyces sp. UR32 using ISP2 medium exhibited anti-HCV activity with IC50 of 4.5±0.22, 3.8±0.18 and 5.7±0.15µM, respectively. Metabolomic analysis of twelve extracts of Spongia irregularis associated actinomycetes led to identification of large number of secondary metabolites. Morever, investigation of cytotoxic and antiviral activities of the extracts revealed that only three extracts exhibited antiviral activity and 7 extracts exhibited cytotoxic activity.
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