In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines.

Elsayed Ahmed Elsayed,Mahmoud A Sharaf-Eldin,Mohammad Wadaan

doi:10.7314/apjcp.2015.16.11.4671

Elsayed Ahmed Elsayed, Mahmoud A Sharaf-Eldin + Show 1 more

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https://doi.org/10.7314/apjcp.2015.16.11.4671

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Abstract

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the IC50 values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and 751.9 μg/mL, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

Highlights

Cancer is one of the most harmful diseases leading to human death in developing as well as developed countries
M. oleifera has been widely investigated for its anticancer activity, scarce information can be found in the literature regarding the cytotoxic activity of its seed essential oil
The present investigation has been carried out to evaluate the antiproliferative activity of M. oleifera seed oil on different cell lines; i.e. HeLa, HepG2, MCF-7, CACO-2 and L929

Summary

Introduction

Cancer is one of the most harmful diseases leading to human death in developing as well as developed countries. Breast and cervical cancers are two of the most common cancer causes in women worldwide (WCR, 2008; Farshori et al, 2013; Srisuwan et al, 2014). In Saudi Arabia, there was a significant increase in the age-standardized incidence rate (ASIR) female breast cancer between 2001 and 2008, with the eastern province recording the highest overall ASIR, at 26.6 per 100,000 women (Alghamdi et al, 2013). The occurrence of cervical cancer in Saudi Arabia is very low, ranking 11th between all female cancers, and accounts only for 2.4% of all new cases reported (Alsbeih et al, 2013). The third most common cancer worldwide, accounts for the fourth occurring cancer among all age groups in Saudi Arabia (Al-Kuraya et al., 2006). In Saudi Arabia, liver cancer accounts for about 10 and 4% of total cancers in males and females, respectively (Al-Ahmadi and Al-Zahrani, 2013)

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