Abstract
The metabolism of 4- 14C-oestradiol-17β in preparations of human myometrium was investigated. The steroid tissue ratio was between 10 3 and 10 6. Minced uterus, brei and homogenate preparations were incubated with oestradiol at 37°C for a period of 30–90 min with and without addition of cofactors. After purification by solvent partition in methanol-petroleum-ether-water the steroids were isolated by column gel chromatography on Sephadex LH-20. The metabolites were identified by their behaviour on gel column, thin-layer and gas chromatography, by radio gas chromatography and by calculation of constant activity in eluates obtained from gel column chromatography on Sephadex LH-20. Under all the experimental conditions employed oestradiol- 17β was metabolized to oestrone, 16-epioestriol, oestriol, 16α-hydroxyoestrone and 16-keto-oestradiol.
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