Abstract
1. 1. The metabolism of [4-C 14]-progesterone in minced uterus, brei and homogenate preparation of human myometrium has been investigated with and without addition of cofactors using a steroid-tissue ratio between 1:1000 and 1:834,000. 2. 2. After purification of the extracts on Amberlite XAD-2 or by solvent partition, the metabolites or groups of metabolites were separated by thin-layer chromatography or gel column chromatography on Sephadex LH-20. 3. 3. The metabolites were identified by their behaviour on thin-layer, gel column and gas chromatography, and by radio gas chromatography or mass spectrometry. 4. 4. Progesterone was metabolized under all the experimental conditions employed in this work; the following metabolites have been characterized or identified: 5α-pregnane-3,20-dione, 20ξ-hydroxy-5α-pregnan-3-one, 20α-hydroxy-4-pregnen-3-one, 6β-hydroxy-progesterone, 5a-pregnane-3β,20α-diol and 5α-pregnane-3α, 20α-diol. The possible formation of other unidentified metabolites is discussed.
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