Abstract
Summary Metabolism of [ 14 C] gibberellin(GA) 12 -aldehyde and [ 14 C]GA 12 in vegetative tissue of potato ( Solanum tuberosum ssp. andigena ) was studied by supplying solutions of these compounds to the base of isolated shoot tips and analyzing the metabolic products by radiodetection after HPLC. Endogenous gibberellins that co-eluted with the 14 C-metabolites on HPLC were identified by GC-MS as GA 53 -aldehyde, GA 53 , GA 44 , GA 19 , GA 20 , GA 29 , GA 1 , and GA 8 . Thus the early-13-hydroxylation pathway seems to be a major pathway in vegetative potato shoots. This was confirmed by time-course feeding studies; according to HPLC elution profiles, the members of the pathway were produced from [ 14 C]GA 12 -aldehyde and [ 14 C]GA 12 in the expected time sequence. The formation of GA 53 appeared to be predominantly via GA 12 rather than GA 53 -aldehyde. The detection of endogenous GA 51 suggests that the non-hydroxylation pathway was also active. Comparisons of HPLC retention times of radioactive metabolites before base hydrolysis with retention times after base hydrolysis showed that potato shoots can conjugate several GAs. HPLC retention times of other radioactive metabolites corresponded with previously published retention times of GA 29 -catabolite, GA 51 -catabolite, GA 12 -aldehyde-conjugate, and GA 53 -aldehyde-conjugate.
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