Abstract
The primary culture of kidney cells from vitamin D deficient chicks is described. After four days in culture the cells reach confluency and retain their ability to metabolize 25-hydroxyvitamin D 3 to 1,25-dihydroxyvitamin D 3. Addition of one unit of bovine parathyroid hormone to the culture medium for 48 hours prior to assay had no effect on the cells' ability to produce 1,25-dihydroxy vitamin D 3, whereas after 24 hours in the presence of 5×10 −8 M 1,25-dihydroxyvitamin D 3 the cells produced not this metabolite, but 24,25-dihydroxyvitamin D 3. This cell culture system will allow the investigation of the regulation of renal 25-hydroxyvitamin D 3 metabolism under controlled in vitro conditions.
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