Abstract
The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC 50 values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC 50 values (46.84 ± 1.83–32.99 ± 2.21 μg/ml) comparing to the standard inhibitors and lower IC 50 value than α-asarone (65.16 ± 2.37–42.15 ± 2.45 μg/ml).
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