Abstract
Abstract Chemical mutagenesis approach was used for the improvement of H 2 yield using Thermoanaerobacterium thermosaccharolyticum . A H 2 yield of 3.12 mol mol −1 glucose was observed which was about 13.5% higher as compared to wild type. An inverse relation was observed between the NADH/NAD + ratio and the rate of H 2 production. Bioelectrochemical analysis showed an overall increase in reducing equivalent in mutants. Continuous H 2 production with mutant using starchy wastewater showed maximum H 2 production rate of 500 mL L −1 h −1 at a dilution rate of 0.25 h −1 and organic loading rate (OLR) of 8.75 g L −1 h −1 . The spent medium was mostly comprised of acetate (0.62 ± 0.3 g L −1 ), butyrate (1.26 ± 0.34 g L −1 ) and ethanol (0.10 ± 0.02 g L −1 ). It was subsequently used as substrate for the biomethanation process to improve overall gaseous energy recovery. Highest methane production rate, methane yield and COD removal of 51.4 ± 0.4 mmol L −1 d −1 , 82.6 L kg −1 COD added , and 66%, respectively were observed at an OLR of 14 g L −1 d −1 thereby improving overall gaseous energy recovery by 53.6%.
Published Version
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