Abstract
Early stages in the infection of chick embryo cells with Rous sarcoma virus (RSV) were examined. Inhibition of protein synthesis with cycloheximide prior to and for a short interval after infection had little effect on subsequent transformation or virus yield, supporting an earlier conclusion ( Bader, 1966) that preexisting enzymes were used for the synthesis of viral DNA. Inhibition of virus reproduction after addition of 5-iododeoxyuridine (IdUrd) for various brief intervals after infection demonstrated that viral DNA synthesis is initiated within the first hour after exposure of cells to virus. Also, as the interval between infection and the addition of IdUrd increased, inhibition of progeny decreased, and the extent of inhibition suggested that viral DNAs accumulate logarithmically. A model is proposed for the residence and function of virus replicative genomes.
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