Metabolic pathways reveal the effect of fungicide loaded metal-organic frameworks on the growth of wheat seedlings

Abstract

Metal-organic frameworks (MOF) are an emerging class of hybrid inorganic-organic porous materials used in various fields, especially in molecule delivery system. As iron is an essential micronutrient for plant growth, iron-based MOF (Fe-MOF) is developed for agricultural application as fungicide carriers. However, fungicides may have various effect on the plant growth, which may be different from Fe-MOF. When they are combined with the carriers, the effects on target plants will change. In this work, tebuconazole-loaded Fe-MOF was prepared and used to treat wheat seedlings. The physiological, biochemical and metabolic levels of wheat roots and shoots were shown by a comparative study. Related metabolic pathways were analyzed by non-targeted metabolomic method. Many metabolites in wheat roots and shoots showed an upward trend after Fe-MOF treatment, but tebuconazole had a negative impact on these indicators. Related metabolic pathways in Fe-MOF and tebuconazole treatment were different, and the related pathway of tebuconazole-loaded Fe-MOF was closer to that of Fe-MOF. The metabolic pathways study revealed that the negative impact from tebuconazole was mitigated when wheat seedlings were treated with tebuconazole-loaded Fe-MOF. This research firstly explores the mechanism of MOF as carriers to help plant reduce the negative effects from fungicide by regulating metabolic pathways.