METABOLIC MARKERS OF LIVER PATHOLOGIES IN FISH

Abstract

Metabolic syndrome is a complex of metabolic, hormonal and clinical disorders. The aim of the research was to consider the main markers of metabolic disorders in the body in fish on a model of toxic damage in zebrafish (20 males and 20 females) zebrafish aged 2-3 months. Before the start of the experiment, the fish were divided into two parity groups. Indicators were monitored daily according to standard methods. The quality of the habitat corresponded to GOST 15.372 - 87, the displacement of the aquarium was 75 liters.The conditions of keeping and feeding (crushed Tetra food) in the experimental group were similar, however, in order to simulate toxic liver damage, Carbamate MN (Moscow) was added to the fish habitat at a concentration of 4.5 mg/l in terms of the active substance.On the 7th day of the experiment, blood was taken from the gill vein in the fish of the experimental and control groups, according to the method of Jill M Murtha (2003), in order to determine biochemical parameters according to the standard method [15,16]. The quantitative indicators revealed during the experiments were processed using the Statistica 6.0 software package.Statistically significant were the indicators of glucose, bilirubin and total protein in individual groups. So the content of glucose and cholesterol is doubled in males and females in the experimental group, compared with the control. The content of bilirubin is higher than the maximum allowable level of this indicator (reference values) by 16%.The use of Carbamate MN in a toxic dosage contributed to metabolic disorders, manifested by a change in the parameters of protein (total protein), fat (cholesterol), carbohydrate (glucose) and pigment (bilirubin) metabolism, which are predictors of the morpho -functional state of the liver.