Abstract

Summary Properties of PEP-carboxylase were followed together with other physiological features of Crassulacean Acid Metabolism (CAM) along the diurnal CAM cycle. There was a significant diurnal change in the susceptibility of PEP-carboxylase against inhibition by L(-)malate. Inhibition was lowest during the night when the phyllodia showed net dark CO, fixation and accumulated malic acid in vacuoles, and inhibition was highest during the day when malate was released from vacuoles, i. e. when the phyllodia deacidified. This changing sensitivity of PEP-carboxylase against inhibition by malate seems to be a property of the native enzyme rather than to be caused by the extraction procedure.

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