Abstract
Summary Habituated cells of Catharanthus roseus (Apocynaceae) selected from an auxin-dependent line accumulated alkaloid in higher amounts than did the non-habituated cells grown in their maintenance medium. Ajmalicine and serpentine contents could be enhanced in both cell lines by feeding the indole alkaloid precursor secologanin or low concentrations of IAA or NAA. However the production remained low in the habituated cells as compared to that of non-autotrophic cells grown in a hormone-free production medium. By comparing the evolution of pH values, the levels of some cellular components and the activities of strictosidine synthase and tryptophan decarboxylase in habituated cells and non-habituated cells grown either in their maintenance medium (with auxin) or in a hormone-free medium, we present evidence that enhancement of alkaloid production in auxin depleted cells might be due to a combination of several factors including a better coordination between tryptamine and secologanine supplies and an increase of vacuolar accumulation capacities.
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