Metabolic Alterations in Male-Sterile Potato as Compared to Male-Fertile.

Maria Shishova,Roman Puzanskiy,Roman Puzanskiy,Galina Pendinen,Olga Gavrilova,Kirill Demchenko,Vladislav Yemelyanov,Alexey Shavarda,Tatjana Gavrilenko,Shamuhommed Kurbanniazov

doi:10.3390/metabo9020024

Abstract

The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids.

Highlights

IntroductionThis type of reproduction provides a source of genetic variation and generates material for adaptation to a rapidly changing environment
Application of the GC-MS method led to an estimation of 193 compounds in the obtained profiles of potato anthers at the mature pollen stage
The number of alterations was found in a pool of fatty acids and lipophilic compounds. This phenomenon is accompanied by a change in the morphology of cell walls

Summary

Introduction

This type of reproduction provides a source of genetic variation and generates material for adaptation to a rapidly changing environment. There are plant species that can reproduce asexually through vegetative propagation. Asexual reproduction produces individuals genetically identical to the parent offspring, a feature which is beneficial under stable conditions. The common potato, Solanum tuberosum, is the fourth most important agricultural crop worldwide. It is able to reproduce both sexually and asexually, though until recently, vegetative propagation by tubers has been the main method of cultivation

Methods

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Discussion

Conclusion