Abstract
The authors studied microbial composition and metabolic activity of the intestinal microbiota in 20 babies born by caesarean section (main group) and 20 babies born vaginally (control group). There were isolated bifidobacteria from meconium at a concentration of 105– 1010 CFU/g by the bacteriological method in 4 (20%) children of the main group and 6 (30%) children in the control group. The gas-liquid chromatography revealed the metabolic activity of various representatives of the intestinal microflora in children of both groups, regardless of the mode of delivery; the indicators of metabolic activity were significantly lower than in the infants. The children in both groups had similar absolute and relative content of short-chain fatty acids in meconium. The data of gas-liquid chromatography indicate the metabolic activity of the meconium microbiota, which is dominated by anaerobic microbial populations and there is a high proportion of proteolytic and conditionally pathogenic flora. The data obtained confirm the process of intrauterine translocation of microbes from mother to child and indicate the importance of the microflora of the future mother in the process of forming the intestinal microbiota of the child.
Highlights
The authors studied microbial composition and metabolic activity of the intestinal microbiota in 20 babies born by caesarean section and 20 babies born vaginally
The gas-liquid chromatography revealed the metabolic activity of various representatives of the intestinal microflora in children of both groups, regardless of the mode of delivery; the indicators of metabolic activity were significantly lower than in the infants
The data of gas-liquid chromatography indicate the metabolic activity of the meconium microbiota, which is dominated by anaerobic microbial populations and there is a high proportion of proteolytic and conditionally pathogenic flora
Summary
Когортное проспективное исследование проводилось на базе отделения новорожденных роддома им. В исследование были включены здоровые доношенные дети (срок гестации 37–40 нед) с массой тела при рождении не менее 2900 г и оценкой по шкале Апгар 7–10 баллов. Основную группу составили 20 детей, рожденные путем кесарева сечения, которое проводилось с целью профилактики несостоятельности рубца на матке после предыдущих оперативных родов, а также при ягодичном предлежании плода. В контрольную группу вошли 20 детей, рожденных путем вагинальных родов. Группы были сопоставимы по характеру течения антенатального и перинатального периодов и состоянию здоровья матерей и новорожденных детей Определение спектра короткоцепочечных жирных кислот в меконии проводили методом газожидкостной хроматографии. При бактериологическом исследовании мекония рост микрофлоры не выявлен у 30 (75%*) детей. Метаболическая активность кишечной микрофлоры у новорожденных детей при различном способе родоразрешения. Характеристика детей, рожденных путем кесарева сечения (основная группа) и вагинальных родов (контрольная группа) Table 2. Characteristics of children born by caesarean section and vaginal birth
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call