Abstract
AbstractStable immobilization and reversible electrochemistry of cytochrome c in a transparent indium tin oxide film with a well‐defined mesoporosity (mpITO) is demonstrated. The transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of electroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbation of the structural integrity of the redox protein. The potential of this modified material as a biosensor for detection of superoxide anions is also demonstrated.
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