Abstract
The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.
Highlights
In the pathogenesis of multiple organ dysfunction syndrome (MODS), the gut has been identified as the central organ [1,2,3]
Histomorphology of the Lung, Intestine, and Liver. Both pulmonary interstitial edema and inflammatory cell infiltration were present in the lungs of the sepsis group, but the degree of injury was ameliorated in the Sepsi s+mesenteric lymph drainage (MLD) group (Figures 1(c) and 1(d))
Despite the significant difference in the grade of lung injury between the sepsis+MLD and control groups, the histomorphological lesions in the lungs of the colon ascendens stent peritonitis (CASP) sepsis model-induced groups were attenuated by mesenteric lymph drainage
Summary
In the pathogenesis of multiple organ dysfunction syndrome (MODS), the gut has been identified as the central organ [1,2,3]. These views are supported by studies in rats subjected to trauma (laparotomy) and hemorrhagic shock, which have shown that the gut-derived inflammatory factors contained in the mesenteric lymph, rather than those in the portal vein, were the cause of lung injury [6].
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