Mesenchymal stem cells and endothelial progenitor cells differentiate into endothelially seeded cells for tissue engineering of heart valve

Abstract

Objective To differentiate mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs),and compare them used as the seeded cells of tissue engineering heart valves. Methods The canine bone MSCs (BMSCs) and EPCs were separated, expanded and differentiated into endothelial cells.The induced BMSCs and EPCs were cultured, expanded, purified, frozen and resuscitated. The BMSC and EPC-derived endothelial cells were examined in cell morphology, proliferation capability, adhering capabili-ty and adhesion onto detergent acellularized porcine valves. The capability of cell resuscitation was studied between the two kinds of endothelial cells. Results Both BMSC and EPC-derived endothelial cells were adhesive growth cells,and spindle in shape. The double time of the BMSC-and EPC-derived endothelial cells was about 34 h and 35 h, respectively. There was no significant difference in adhering capability and cell resuscitating rate. Scanning microscopy revealed that the two kinds of ceils could be planted on the de-tergent acellularized porcine-valves. Conclusion BMSCs and EPCs can be induced to differentiate into endothelial cells,and can be used as seeded cells to fabricate tissue engineered heart valves. Key words: Mesenchymal stem cells; Endothelial progenitor cells; Valve; Seeded cells; Tissue engineering