Mercury determination in work place air and human biological samples based on dispersive liquid-liquid micro-extraction coupled with cold vapor atomic absorption spectrometry

Abstract

Mercury as a Heavy metal is important factor must be determined and controlled in environmental air and biological. Mercury (Hg) accumulate in living tissues of human body. By NIOSH method, the briefing work place air of petrochemical worker was measured by cold vapor atomic absorption spectrometry(CV-AAS). For preparation and preconcentration samples before analysis a new method of dispersive liquid-liquid micro-extraction with ammonium salt of 1-pyrrolidinedithiocarbamate(APTC)combined with cold vapor atomic absorption spectroscopy, was developed.The influences of various analytical parameters including pH, APDC concentration and ionic liquid volume were investigated.Quantitative recoveries were obtained at pH 7.The enrichment factor was calculated as 5.The detection of limit(LOD)and detection of quantification(LOQ)of mercury were 0.06 and 0.2 μg L-1 respectively. In order accuracy determination the method,certified reference materials, NIST SRM 3133 Lot 061204 was analyzed and the recovery value 98% obtained. So, method of DLLME-APTC had good potential for preparation and preconcentration mercury samples before analysis.