Abstract
Simple sequence repeats (SSRs) markers are important molecular tools widely used in genetic and genomic research in plants. However, the number of SSR markers developed in lentils (Lens culinaris Medik.) so far, which is an economically important cool season legume plant, has been very limited. The lack of SSR markers is among the main factors limiting lentil molecular breeding studies. In this study, 288 clones belonging to the enriched libraries with AC and AG repeats were screened by TG and TC repeats to develop SSR markers in lentil plants and 15 new SSR markers were developed. A total of 18 alleles were generated from markers showing polymorphism, and the Lc-MCu54 marker was identified as the most polymorphic marker. These developed markers are capable of contributing to many molecular-based studies in lentil plants.
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