Abstract
This work describes EtOH,O2 membraneless enzymatic biofuel cells (EtOH,O2 MlessEBFCs) that employ laccase-based biocathodes and ADH/NAD+ bioanode. Laccase biocathodes were prepared by immobilizing a polypyrrole film containing different redox mediators (ruthenium and osmium complexes). The bioanode for EtOH,O2 MlessEBFCs was fabricated by immobilizing multiwalled carbon nanotubes, NAD+-dependent alcohol dehydrogenase enzyme (ADH), poly-methylene green, and poly(amidoamine) (PAMAM) dendrimer onto a carbon cloth platform. Maximum power density and current density were 21.0 ± 0.2 mW cm-2 and 0.15 ± 0.07 mA cm-2, respectively, in PBS (pH 6.5). Lifetime tests conducted for EtOH,O2 MlessEBFCs showed promising perspectives for their future application in miniaturized devices.
Highlights
Biofuel cells (BFCs) employ enzymes or microorganisms as catalysts to convert chemical energy into electric energy
The bioanode for EtOH,O2 MlessEBFCs was fabricated by immobilizing multiwalled carbon nanotubes, NAD+-dependent alcohol dehydrogenase enzyme (ADH), polymethylene green, and poly(amidoamine) (PAMAM) dendrimer onto a carbon cloth platform
A 20-day lifetime has been reported for a membraneless ethanol, oxygen enzymatic biofuel cell (EtOH,O2 MlessEBFC) based on alcohol dehydrogenase (ADH) and bilirubin oxidase (BOD) as bioelectrodes[5]
Summary
Biofuel cells (BFCs) employ enzymes or microorganisms as catalysts to convert chemical energy into electric energy. To prepare MlessBFCs successfully, enzyme immobilization is a key step to obtain a stable longlasting device, improving electron transfer kinetics, and increasing power densities (PDs). In this context, researchers have sought to enhance enzymatic system robustness and activity—an enzymatic system must be able to survive pH, temperature, and reaction medium changes. Strategies to enhance ET between enzymes and electroactive surfaces include orientation and immobilization of the enzymes and electron mediation For this laccase-based biocathode metallic redox complexes (Os and Ru) was entrapped in a polyPYR film as redox mediators and the ADH/NAD+ bioanode employed polymethylene green layer as mediator. We investigate the activity of the membraneless biofuel for a long period (11 months) in other to show their stability
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