Abstract
BackgroundThis experiment is conducted to evaluate the anti-inflammatory effect of Piper chaba roots.MethodsThe in-vitro anti-inflammatory activity of Piper chaba was carried out by human red blood cell (HRBC) membrane stabilization method which includes heat-induced hemolysis and hypo tonicity- induced hemolysis and also by another method of egg albumin denaturation assay.ResultsAnti-inflammatory activity study of crude ethanolic extract was performed using heat induced membrane stabilization method, hypo-tonicity induced HRBC membrane stabilization method and egg albumin denaturation method. Crude ethanolic extracts of P. chaba showed promising in vitro anti-inflammatory activity in a concentration dependent manner. Using acetyl salicylic acid (ASA) as standard drug and was compared with ethanolic extract to determine anti-inflammatory activity. Heat induced anti-inflammatory test revealed that crude ethanolic extract of P. chaba (500 μg/ml) and positive control ASA(500 μg/ml) have 52.667% and 78% respectively, hypo tonicity induced anti-inflammatory test showed 35.67% and 59% inhibition of red blood cell (RBC) hemolysis. Egg albumin denaturation method also evaluated that crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) showed 60% and 97.12% inhibition of egg albumin denaturation.ConclusionThe plant of P. chaba of the genus Piper possesses promising anti-inflammatory activities.
Highlights
The use of natural products having therapeutic activities is an ancient practice for human civilization and plants, minerals and animal products (Epibatidine, African clawed frog) were the primary sources of drugs since ancient time [1]
Natural compounds that are rectified of plants may suffice as very good templet for discovery and design of novel anti-inflammatory lead molecules and drugs with less toxicological status because gastrointestinal problems or liver cirrhosis associated with the use of synthetic antiinflammatory drugs enduring dilemma of medical world [8]
This effect can be demonstrated by heat-induced membrane stabilization method, hypotonicity induced human red blood cell (HRBC) membrane stabilization method and egg albumin antidenaturation method
Summary
The use of natural products having therapeutic activities is an ancient practice for human civilization and plants, minerals and animal products (Epibatidine, African clawed frog) were the primary sources of drugs since ancient time [1]. Using overthe-counter (OTC) with non-steroidal prescription medicaments are frequently commended in case of distinctive neurosurgical practice It is very important processing both analgesic along with anti-inflammatory activities which decidedly can bring upon several novel prospects for inflammation related diseases. Natural compounds that are rectified of plants may suffice as very good templet for discovery and design of novel anti-inflammatory lead molecules and drugs with less toxicological status because gastrointestinal problems or liver cirrhosis associated with the use of synthetic antiinflammatory drugs enduring dilemma of medical world [8]. Piperine compound is isolated from Piper nigrum can cause acute changes in inflammatory processes. Another plant from Piper genus Piper porphyrophyllum (Piperaceae) a compound- 4′,5-dihydroxy-3′,7-dimethoxyflavone gave anti-inflammatory activity [13, 14]. This experiment is conducted to evaluate the anti-inflammatory effect of Piper chaba roots
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