Membrane remodelling and triacylglycerol accumulation in drought stress resistance: The case study of soybean phospholipases A

Abstract

Agriculture is facing major constraints with the increase of global warming, being drought a major factor affecting productivity. Soybean (Glycine max) is among the most important food crops due to the high protein and lipid content of its seeds despite being considerably sensitive to drought. Previous knowledge has shown that drought induces a severe modulation in lipid and fatty acid content of leaves, related to alteration of membrane structure by lipolytic enzymes and activation of signalling pathways. In that sense, little is known on lipid modulation and lipolytic enzymes’ role in soybean drought stress tolerance. In this work, we present for the first time, soybean leaves lipid content modulation in several drought stress levels, highlighting the involvement of phospholipases A. Moreover, a comprehensive analysis of the phospholipase A superfamily was performed, where 53 coding genes were identified and 7 were selected to gene expression analysis in order to elucidate their role in soybean lipid modulation under water deficit. Proportionally to the drought severity, our results revealed that galactolipids relative abundance and their content in linolenic acid decrease. At the same time an accumulation of neutral lipids, mainly due to triacylglycerol content increase, as well as their content in linolenic acid, is observed. Overall, PLA gene expression regulation and lipid modulation corroborate the hypothesis that phospholipases A may be channelling the plastidial fatty acids into extraplastidial lipids leading to a drought-induced accumulation of triacylglycerol in soybean leaves, a key feature to cope with water stress.