Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL7-Receptor Signalosome in Human CD4 T-Cells

Abstract

Event Abstract Back to Event Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL7-Receptor Signalosome in Human CD4 T-Cells BLANCHE TAMARIT1 and THIERRY ROSE1* 1 Institut Pasteur, Infection and Epidemiology/Immunology, France Interleukin(IL)-7 is the main homeostatic regulator of CD4 T-lymphocytes (helper) at both central and peripheral levels. Upon activation by IL-7, several signalling pathways, mainly Jak/STAT, PI3K/Akt and MAPK, induce the expression of genes involved in T-cell differentiation, activation and proliferation. We have analyzed the early events of CD4 T-cell activation by IL-7. We have shown that IL-7 in the first few minutes induces the formation of cholesterol-enriched membrane microdomains that compartmentalize its activated receptor and initiate its anchoring to the cytoskeleton supporting the formation of the signalosome on the IL-7-receptor cytoplasmic-domains [1]. We describe by stimulated emission depletion (STED) microscopy, the key roles played by membrane microdomains and cytoskeleton transient organization in the IL-7-regulated Jak/STAT signalling pathway [2]. We image phospho-STAT5 and cytoskeleton components along IL-7-activation kinetics using appropriate inhibitors. Lipid raft inhibitors delay and reduce IL-7-induced Jak1 and Jak3 phosphorylation. Drug-induced disassembly of cytoskeleton inhibits phospho-STAT5 formation, transport and translocation into the nucleus that controls the transcription of genes involved in T-cell activation and proliferation. We fit together the results of these quantitative analyses and propose the following mechanism sketched in the figure bellow with the corresponding STED images [2]: activated IL-7-receptors embedded in membrane microdomains induce actin-microfilament meshwork formation, anchoring microtubules that grow radially from rafted receptors to the nuclear membrane. STAT5 phosphorylated by signalosomes are loaded on kinesins and glide along the microtubules across the cytoplasm to reach the nucleus two minutes after IL-7-stimulation. Radial microtubules disappear 15 minutes later while transversal microtubules, independent of phospho-STAT5 transport, begin to bud from the microtubule-organization-center. Figure 1 References [1] T. Rose, A. H. Pillet, V. Lavergne, B. Tamarit, P. Lenormand, J.C. Rousselle, A. Namane, J. Thèze, Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response, J Biol Chem, 285, 14898, 2010. [2] B. Tamarit, F. Bugault, A. H. Pillet, V. Lavergne, P. Bochet, N. Garin, U. Schwarz, J. Thèze, T. Rose, Membrane microdomains and cytoskeleton organization shape and regulate the IL7-receptor signalosome in human CD4 T-cells, J Biol Chem, 288 Keywords: lipid microdomain, Cytoskeleton, Interleukin-7, JAK/STAT signaling pathway, human CD4 T-cell, Kinesin Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: TAMARIT B and ROSE T (2013). Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL7-Receptor Signalosome in Human CD4 T-Cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00028 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. THIERRY ROSE, Institut Pasteur, Infection and Epidemiology/Immunology, Paris, 75015, France, rose@pasteur.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers BLANCHE TAMARIT THIERRY ROSE Google BLANCHE TAMARIT THIERRY ROSE Google Scholar BLANCHE TAMARIT THIERRY ROSE PubMed BLANCHE TAMARIT THIERRY ROSE Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.