Fluorescence nanoscopy of single DNA molecules by using stimulated emission depletion (STED).

F Persson,P Bingen,J O Tegenfeldt,J Engelhardt,T Staudt,Stefan W Hell

doi:10.1002/anie.201100371

Abstract

Fluorescence nanoscopy of single DNA molecules by using stimulated emission depletion (STED).

Highlights

Sample preparation: Coverslides were cleaned with methanol and blowdried
The DNA was prepared by mixing λ-bacteriophage DNA (Amersham Biosciences, Buckinghamshire, UK) at a concentration of 10 μg/ml with YOYO-1 (Invitrogen, Carlsbad, USA) in order to obtain the desired basepair:dye staining ratio of 5:1 and 20:1
Prior to the experiments this solution of labelled DNA was diluted 10-fold with an imaging buffer consisting of 0.5x TBE buffer, degassed under ultrasonic agitation for 30 min, and 5 v/v% BME

Summary

Introduction

Sample preparation: Coverslides were cleaned with methanol and blowdried. A 50 μl droplet of poly-L-lysine solution Fluorescence Nanoscopy of Single DNA molecules by using Stimulated Emission Depletion (STED)** Sample preparation: Coverslides were cleaned with methanol and blowdried.

Results

Conclusion