Abstract
Our lab has been involved for some time in the development of strategies for assembling and partitioning model membranes on solid supports. While ideally suited for analysis by surface-sensitive methods, the close proximity of the lower leaflet of the supported bilayer to the solid support limits its application, especially for transmembrane proteins. In order circumvent this limitation, we have developed three model membrane architectures in which the bilayer is separated from the support: tethered vesicles using DNA-lipid conjugates which can be used to study vesicle-vesicle interactions and fusion; structures that position a black lipid membrane in close proximity to a highly reflective mirror for interferometry in combination with electrical measurements; and membrane patches tethered to solid supports or to fluid supported bilayers using DNA-lipid conjugates. Each architecture offers specific advantages and opportunities, and recent results will be described.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
