Abstract
Association between the membrane and replication origins of the Bacillus subtilis chromosome has been examined during synchronous dichotomous replication where two successive initiations result in four copies of the replication origin and one copy of the terminus per chromosome. The four origins can be distinguished from each other by a combination of density transfer experiments, pulse label at the origin and hybridization with the separated strands of B. subtilis DNA. The data indicate that, when the chromosome contains four replication origins, all four are associated with the membrane.
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