Abstract
Cucurbit yellow stunting disorder virus (CYSDV) is one of the main limiting factors of melon cultivation worldwide. To date, no commercial melon cultivars resistant to CYSDV are available. The African accession TGR-1551 is resistant to CYSDV. Two major quantitative trait loci (QTLs) have been previously reported, both located near each other in chromosome 5. With the objective of further mapping the gene or genes responsible of the resistance, a recombinant inbred line (RIL) population derived from the cross between TGR-1551 and the susceptible cultivar ‘Bola de Oro’ was evaluated for resistance to CYSDV in five different assays and genotyped in a genotyping by sequencing (GBS) analysis. The major effect of one of the two QTLs located on chromosome 5 was confirmed in the multienvironment RIL assay and additionally verified through the analysis of three segregating BC1S1 populations derived from three resistant RILs. Furthermore, progeny test using the offspring of selected BC3 plants allowed the narrowing of the candidate interval to a 700 kb region. The SNP markers identified in this work will be useful in marker-assisted selection in the context of introgression of CYSDV resistance in elite cultivars.
Highlights
The Cucurbit yellow stunting disorder virus (CYSDV) is a Crinivirus of the family Closteroviridae [1,2]
The work reported has allowed the narrowing of the candidate interval for the major QTL associated with resistance to CYSDV derived from TGR-1551 to a region of approximately 700 kb
The SNP markers provided here are useful in marker-assisted selection (MAS) in breeding programs aimed at the introgression of CYSDV resistance
Summary
The Cucurbit yellow stunting disorder virus (CYSDV) is a Crinivirus of the family Closteroviridae [1,2]. CYSDV was first reported in the United Arab Emirates [3] and subsequently spread throughout the Middle East and the Mediterranean Basin [4,5,6,7,8,9,10], North and Central America [9,11,12,13], and China [14] Nowadays, this virus has become a significant threat throughout the cucurbit production regions in the southern United States, Mexico, and Central America [15] and has been reported as the most economically important of the viruses affecting cucurbit production in the southwestern United States [16]. Artificial infection methods such as mechanical inoculation or agroinfiltration have not been successful
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
