Melatonin protects against hyperglycemia-induced Schwann cells apoptosis through MTOR and Wnt signaling pathways

Abstract

Event Abstract Back to Event Melatonin protects against hyperglycemia-induced Schwann cells apoptosis through MTOR and Wnt signaling pathways Yee Lian Tiong1, Soi Moi Chye2, Rhun Yian Koh2, Ponnudurai Gnanajothy2 and Khuen Yen Ng3* 1 International Medical University, School of Postgraduate Studies and Research, Malaysia 2 International Medical University, Department of Human Biology, Cell and Molecules, Malaysia 3 Monash University, Jeffrey Cheah School of Medicine and Health Sciences, Malaysia Diabetic neuropathy is a commonly reported complication of diabetes mellitus. The current treatment regime could not effectively treat diabetic neuropathy, which is a debilitating condition that approximately afflicts 70% of the patients. Diabetic neuropathy affects both autonomic and peripheral nerves, leading to severe pain, disability and might even fatal at times. Hence it is very important to identify an adjuvant therapy that can protect or minimize the damage from diabetic neuropathy for diabetes patients. It has been reported that Schwann cells dysfunction and apoptosis due to prolonged exposure to high glucose are important pathological features of diabetic neuropathy. Melatonin is a neurohormone secreted by pineal gland and has been reported with multiple beneficial actions such as antioxidant and anti-inflammatory. Thus the present study investigates the potential protective effects of melatonin effective against Schwann cells damage under hyperglycemic condition and studies the underlying mechanisms. Our results showed that melatonin treatment significantly reduced hyperglycemia-induced cell death and increased cell survival. Cell death due to oxidative stress was measured by DCFH-DA assay. Result revealed that hyperglycemia induced oxidative stress was attenuated by melatonin. Moreover, melatonin prevented hyperglycemia induced-Schwann cells mitochondrial membrane potential depletion and cell apoptosis was detected by flow cytometry. Immunoblotting showed that under hyperglycemia condition, Wnt and its co-receptor (Lrp), mTOR , raptor and rictor were down-regulated. Melatonin could protect hyperglycemia-induced cell death through up-regulated Wnt, Lrp, mTOR, raptor and rictor protein expression. Our conclusion was melatonin protects against hyperglycemia-induced Schwann cells apoptosis through MTOR and Wnt signaling pathways. Keywords: Apoptosis, Melatonin, Schwann Cells, Diabetic neuropathy, Signalling pathway Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Tiong Y, Chye S, Koh R, Gnanajothy P and Ng K (2016). Melatonin protects against hyperglycemia-induced Schwann cells apoptosis through MTOR and Wnt signaling pathways. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00176 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Khuen Yen Ng, Monash University, Jeffrey Cheah School of Medicine and Health Sciences, Bandar Sunway, Selangor, Malaysia, ng.khuen.yen@monash.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yee Lian Tiong Soi Moi Chye Rhun Yian Koh Ponnudurai Gnanajothy Khuen Yen Ng Google Yee Lian Tiong Soi Moi Chye Rhun Yian Koh Ponnudurai Gnanajothy Khuen Yen Ng Google Scholar Yee Lian Tiong Soi Moi Chye Rhun Yian Koh Ponnudurai Gnanajothy Khuen Yen Ng PubMed Yee Lian Tiong Soi Moi Chye Rhun Yian Koh Ponnudurai Gnanajothy Khuen Yen Ng Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.