Abstract
ABSTRACT: Beef tallow was fractionated either without or with solvent to obtain a highly enriched unsaturated fatty acid (USFA) fraction. Solvent fractionation at low temperature (‐18 °C) successfully fractionated USFA‐enriched triacylglycerol (TAG). The higher that the solute to solvent ratio was used for fractionation, the more weight yield of USFA‐enriched TAG from the liquid fractions was recovered during the fractionation. USFA‐enriched fraction was further esterified with lipase and medium chain fatty acid (caprylic acid). Through 2nd‐step transesterification, MLM (sn‐1‐medium‐2‐long‐3‐mediumTAG) type of structured lipids (SL) was produced in volume. Isolated SL was characterized by reverse‐phase HPLC, GC, and DSC. Synthesized SL in this study would be considered as a value‐added product from renewable animal fat for nutritionally beneficial applications in food.
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