Medium supplementation and thorough optimization to induce carboxymethyl cellulase production by Trichoderma reesei under solid state fermentation of nettle biomass

Abstract

In the present study, the production of cellulase by Trichoderma reesei under solid-state fermentation of nettle biomass was promoted through supplementation of the culture media using carbonaceous additives and comprehensive optimization of the cultivation via the Taguchi method. CMCase activities about 5.5–6.1 U/gds were obtained by fermentation of the autoclave-pretreated biomass, among various chemical and physical pretreatments. Then, several additives including Tween 80, betaine, carboxymethyl cellulose, and lactose were individually or in combination added to the culture media to induce the enzyme production. The results proved that such additives could act as either inducers or inhibitors. Furthermore, CMCase activity surprisingly increased to 14.0 U/gds by supplementing the fermentation medium with the optimal mixture of additives including 0.08 mg/gds Tween 80, 0.4 mg/gds betaine, and 0.2 mg/gds carboxymethyl cellulose. Factor screening according to Plackett–Burman design confirmed that the levels of Urea and MgSO4 among basal medium constituents as well as pH of the medium were significantly affected CMCase production. By optimizing the levels of these factors, CMCase activity of 18.8 U/gds was obtained, which was noticeably higher than that of fermentation of the raw nettle. The applied procedure can be promisingly used to convert the nettle biomass into valuable products.