Abstract
Implementation of non-invasive PGT-A (niPGTA) requires avoiding DNA contamination from cumulus cells or other embryos. This requires adjustment of culture protocols to optimize niPGTA results while not compromising embryo development. One approach entails refreshing culture medium on day 4, after the maternal-zygotic transition, before individual culture to avoid DNA contamination issues. The objective of this study was to compare the impact of day 3 or a day 4 media refresh on resulting embryo development.
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