Abstract
The ZnO, ZnO-Eugenol complex (ZnO-EG) and Ag decorated ZnO-Eugenol complex composite (0.5, 1.0% Ag-ZnO-EG) materials were effectively prepared by mechanochemical process. The synthesized materials were fully characterized by TG-DTA, FTIR, XRD and SEM-EDS characterization techniques. The granular nanocrystallites of ZnO were found to be transformed into fine porous bricks like morphology in ZnO-EG complex and 0.5, 1.0% Ag-ZnO-EG composites. All the as synthesized materials were evaluated for their significant antibacterial activities in order to identify their MIC values and zone of inhibition studies against four different bacterial species viz. Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The mode of bactericidal activity shown by highest active 1.0% Ag-ZnO-EG composite was investigated on the basis of SEM analysis of the treated cells. The dual mode of action of the studied material illustrated direct pore formation on the cell surface of Gram negative Escherichia coli species whereas in Gram positive Bacillus subtilis the agglomeration of the composite on bacterial surface was spotted. The Ag decoration in ZnO-EG complex was found to be crucial to exhibit significant bactericidal activity in Ag-ZnO-EG composites.
Published Version
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