Abstract
Type III-A CRISPR-Cas complex, Csm, plays a pivotal role in the small RNA-mediated immunity in bacteria. In the presence of a cognate target RNA (CTR) representing a viral RNA, Csm cleaves CTR, and in addition, elicits single-stranded deoxyribonuclease (ssDNase) and cyclic oligoadenylate (cOA) synthetase activities. In contrast, in the presence of a non-cognate target RNA (NTR) that represents self-RNA, the two collateral activities are inhibited. To explain how RNA stimulate Csm activity, we reconstituted Csm from Lactococcus lactis, LlCsm, and characterized its structure and function.
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