Mechanism research of matrine in the progress of prostate cancer

Abstract

Objective To clarify the mechanism of matrine in the progress of prostate cancer. Methods (1) Gene sequencing identifies the differentially expressed genes between matrine-treated and untreated PC3 and DU145. (2) The apoptotic pathway and prostate cancer related genes were selected according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and TCGA database. (3) Real-time Quantitative PCR Detecting System (RT-qPCR)verifies the effect of matrine on the expression of DNA damage inducible beta(GADD45B) and B cell lymphoma/leukemia-2 (bcl-2) like 11 (BCL2L11). (4) Set four groups: DU145 (blank, NC), DU145+ overexpression GADD45B (experimental group 1, GADD45B), DU145+ matrine (experimental group 2, Matrine) and DU145+ matrine + overexpression GADD45B(experimental group 3, Matrine+ GADD45B). Wound healing, cell counting kit-8 (CCK-8), transwell and flow cytometry experiment were performed to detect the migration, viability, invasion and apoptosis respectively. Results (1) There were 1 240 differentially expressed genesbetween PC3 and DU145 cell lines after treated withmatrine. (2) 2 of them were related to apoptosis and prostate cancer, in which the expression of GADD45B was positively (P=0.024) while the bcl-2 like 11 (BCL2L11) was negatively (P=0.015) correlated with the survival rate of patients. (3) Matrine promoted the expression of GADD45B (P=0.014 in DU145; P=0.002 in PC3) and BCL2L11 (P=0.000 in two cell lines). (4) Both matrine and GADD45 could inhibit cell proliferation, migration and invasion, and promote cell apoptosis. Conclusion Matrine inhibits the progression of prostate cancer by promoting the expression of GADD45B. Key words: Matrine; DNA damage inducible beta; Prostate cancer