Abstract
Reactions of different phosphates (represented here as PO4), including H2PO4–(as prototype), phenylphosphate (and the p-nitro derivative), pyrophosphate, tripolyphosphate, and adenosine 5′-triphosphate (ATP), with the FeIIFeIII form of purple acid phosphatase (PAPr) from porcine uteri (uteroferrin) have been studied by monitoring absorbance changes for the iron(III) chromophore at 620 nm. Stopped-flow rate constants are independent of total [PO4](10–50 mM), and decrease with increasing pH (2.5–6.5). At the lower pH a mechanism of rapid PO4 binding to the FeII, followed by rate-controlling [PO4]-independent bridging to the FeIII with displacement of a co-ordinated H2O, is proposed. Further information comes from experiments on the hydrolysis activity of PAPr monitored by the release of α-naphthol (323 nm) from α-naphthyl phosphate, which maximises at pH 4.9. The full mechanism requires participation of FeIII–OH, which substitutes into the phosphate moiety thus bringing about hydrolysis. The concentration of the latter peaks at pH 4.9, and possible reasons for the decrease in activity at pH >4.9 are given. Rate constants at maximum activity are of magnitude ≈ 0.5 s–1 only, with no very strong discrimination between the reagents used. Equilibration steps in which the phosphate can if necessary be recycled to bring about hydrolysis are proposed. For the pH range studied the final product has a bridging HPO42– ligand. Trimethyl phosphate with only one oxo group does not appear to react at the FeIII, but inhibits reaction with H2PO4– possibly by co-ordinating to the FeII. Reaction with the sterically bulky cation [Co(NH3)6(HPO4)]+ is much slower, k= 1.6 × 10–4 s–1. The HPO4–-bridged FeIIFeIII form is more responsive to air oxidation to FeIIIFeIII consistent with the decrease in reduction potential from 367 and 183 mV. Rate constants are independent of [H2PO4–] and pH.
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More From: Journal of the Chemical Society, Dalton Transactions
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