Abstract
It has been shown that the ATP-dependent incorporation of [ 14C]serine into phosphatidylserine in rat liver mitochondrial and microsomal fractions is prevented by EGTA. On the other hand, at low (μM) Ca 2+ concentrations, serine incorporation is strongly stimulated by ATP and Mg 2+. This stimulatory effect is reduced by calcium ionophore A23187. It is therefore suggested that the ATP-dependent process is that of serine base-exchange reaction, stimulated by endogenous Ca 2+ accumulated inside the microsomal vesicles by Ca 2+,Mg 2+-ATPase. The mitochondrial activity can be accounted for by contamination by the endoplasmic reticulum. Phosphatidylserine; Base-exchange reaction; ATPase, Ca 2+,Mg 2+-; (Endoplasmic reticulum)
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