Mechanism of salvianolate regulating activity of NLR family pyrin domain containing 3 inflammasome in treatment of mice with colitis

Abstract

Objective To study the role of salvianolate in the treatment of inflammation of intestinal mucosa by colitis mice model. Methods A total of 60 C57BL/6J mice were divided into acute control group, acute model group, acute interventional group, chronic control group, chronic model group and chronic interventional group with 10 mice in each group. Dextran sodium sulfate (DSS) was used to establish the model. Meanwhile, the mice of interventional group received salvianolate peritoneal injection during modeling. The expression levels of NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ACS) and cysteinyl aspartate specific proteinase 1 (caspase-1) were detected. And the absorbances of serum superoxide dismutase (SOD) and malonaldehyde (MDA) in mice serum were determined. Kruskal Wallis single factor analysis of variance, single factor analysis of variance, Student-Newman-Keuls (SNK) method and least-significant difference method were performed for statistical analysis. Results The expressions of NLRP3 at protein level of acute control group, acute model group, acute interventional group, chronic control group, chronic model group and chronic interventional group were 9 965.20 (196.81), 16 703.38 (291.21), 13 423.74 (209.28), 10 112.01 (183.55), 16 247.90 (505.44) and 12 674.95 (229.32), respectively. The expressions of NLRP3 of acute and chronic interventional group were both lower than those of acute and chronic model group, and the differences were statistically significant (both χ2=15.158, P 0.05). SOD level of chronic interventional group was higher than that of model group, and the difference was statistically significant (t=-2.65, P=0.021). The serum levels of MDA were (1.31±0.40), (6.95±1.57), (2.98±1.41), (1.21±0.16), (9.38±3.73) and (3.74±0.81) nmol/L, respectively. MDA levels of acute and chronic interventional groups were lower than those of acute and chronic model groups, and the differences were statistically significant (t=4.88 and 4.02, both P<0.01). Conclusion Salvianolate may alleviate oxidative stress response by adjusting the activity of NLRP3 inflammasome which takes part in reducing intestinal inflammation. Key words: Inflammatory bowel diseases; Salvianolate injection; Inflammasome; Oxidative stress; Mouse