Abstract
Poly lactic acid-glycolic acid copolymer (PLGA) plays a critical role in inducing tumor cells apoptosis. However, the solubility of the compound itself puts a limit on its use value in treatment of tumor. In this study, we used PLGA combined with nanoparticles as a strategy for metastatic tumor of lung cancer. We evaluated the biological effect of PLGA nanoparticles on enhancing cytotoxicity and its anti-tumor effect against metastasis tumor of lung cancer. Confocal microscope was used to detect the transfection efficiency of tumor cells. PLGA and PLGA-cored nanoparticles (PLGA AC-NPs) were used to interfere with proliferation and apoptosis of metastatic tumor cell line from lung cancer and flow cytometry was used to explore its effect. Furthermore, PLGA and PLGA AC-NPs were injected into the chest of lung cancer mice for treatment. Cell proliferation and apoptosis were detected through measurement of tumor volume, immunohistochemistry (IHC) and Westernblot protein analysis. It was found that, PLGA AC-NPs significantly enhanced cell uptake while PLGA ACNPs regulated tumor by inhibiting cell proliferation and promoting apoptosis. It was also observed in vivo transplanted tumor model that the growth of metastatic tumor of lung cancer was inhibited significantly by NGO-PEG-PEI/Cer, while proliferation and accelerated apoptosis were also slowed down. PLGA AC-NPs can inhibit tumor growth by promoting apoptosis and inhibiting further proliferation of tumor cells.
Published Version
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