Abstract
The supernatant fraction of Escherichia coli cells treated with [ 14C]-2,3-dihydro-1,3-6H-oxazine-2,6-dione ([ 14C]-3-oxauracil) contains the principal portion of radioactivity in the low molecular weight subfraction. The dominant component of this subfraction is a compound with the properties of 3-oxauridine 5′-monophosphate. The compound is also formed from 3-oxauracil and 5-phosphoribosyl 1-pyrophosphate on incubation with the cell-free extract of E. coli. If this incubation mixture is enriched with adenosine 5′-triphosphate, two further compounds are formed, their properties corresponding to 3-oxauridine 5′-di- and -triphosphates. Treatment of the compound possessing properties of 3-oxauridine 5′-triphosphate with snake venom yielded a non-nucleotide compound with a lower chromatographic mobility than authentic 3-oxauridine. This compound appears to be a ribonucleoside with an open oxazine ring.
Published Version
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