Abstract
Objective To investigate the mechanism of dexamethasone (Dex) in inhibiting monocyte adhesion and phagocytose function. Methods Under the stimulation of phorbo1-12-myristate-13-acetate (PMA),U937 monocytes cultured in vitro were treated with Dex and Fasudil respectively.The adhesion rate of U937 monocles to human umbilical vein endothelial cells (HUVECs) and their phagocytic ability of India ink were studied.The protein content and activity of rho-associated coiled-coil protein kinase 1 ( ROCK1 ) as well as the effects of mifepristone and cycloheximide on Dex were determined.Results Both DEX and Fasudil could significantly inhibit the adhesion tate and phagocytosis of U937 cells stimulated by PMA and suppressed the activity of ROCK1.While mifepristone and cycloheximide could not alter these effects of DEX. Conclusion DEX interferes with the adhesion and phagocytosis function of U937 cells by inhibiting ROCKI activity. Key words: Dexamethasone; Phagocytose; Non-genomic effect
Published Version
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