Abstract
Objective To investigate the mechanism of postoperative anti-scarring effect of retinoic acid (RA) drug delivery system (RADDS) on a rabbit model of glaucoma filtration surgery.Methods Glaucoma filtration surgery was performed on both eyes of 25 New Zealand white rabbits.The left eyes received RADDS (RA + PLGA),15 right eyes only randomly received drug delivery system (PLGA),and remaining 10 right eyes served as null vehicle (NV).The rabbits were killed on the day 7,14,30,60and 90 after the surgery.Both eyes were enucleated and tissue sections were immunohistochemically stained for proliferating cell nuclear antigen (PCNA) and transforming growth factor-52 (TGF-β2) of fibroblasts in surgery sites of rabbits' eyes.Semi-quantification of TGF-β2 mRNA was performed using reverse transcription-polymerase chain reaction (RT-PCR).Results The RA drug delivery system reduced fibroblast activity,with a significant reduction in the number and expression of PCNA in fibroblasts (P ＜ 0.01).RADDS treatment could significantly reduce the expression of TGF-β2 protein and mRNA (P ＜0.01).Conclusion The effect of postoperative anti-scarring of RA drug delivery system was mediated by inhibiting proliferation of fibroblasts and altering the expression of TGF-β2. Key words: Retinoic acid ; Glaucoma filtration surgery; Scarring; Proliferating cell nuclear antigen; Transforming growth factor-β
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