Mechanical properties of outer layers from near-isogenic lines of common wheat differing in hardness

Abstract

The mechanical properties of the combined outer layers from near-isogenic wheats differing by hardness were determined. Results from traction tests showed significant differences between the isogenic lines, outer layers from grains of the soft type showing higher extensibility. Determination of the mechanical properties of the corresponding component tissues revealed significant differences between the isolated tissues from soft or hard wheat grains. It also allowed analysis of their respective contribution to the properties of the combined peripheral tissues using a simulation of their rupture as unseparated tissues. According to the results, if the component layers displayed similar maximum lineic force to rupture, the rupture of combined outer layers occurs when the least extensible individual tissue breaks. The major cell wall biochemical components of the combined outer layers and of their component tissues were analysed. The phenolic acid composition of soft wheat pericarp contained more ferulic acid in either monomeric or polymeric forms than the pericarp from hard wheat. Arabinoxylans in walls of the soft wheat pericarp appeared 1.6 times more cross-linked by ferulic acid dehydrodimers than walls of hard wheat. These differences in arabinoxylan cross-linking may explain the observed differences in pericarp mechanical properties.