Abstract
The force curve measurement mode of the atomic force microscope (AFM) is a powerful experimental technique in biotechnology. However, it is more effective if the spectroscopic properties of the biomolecule in the contact area can be simultaneously measured. Thus, we developed a confocal laser scanning microscope/AFM system. In this study, we simultaneously measured the fluorescence spectra of green fluorescent protein with the application of an external force in order to investigate the stability and dynamics of the β-barrel structure. Consequently, the fluorescence was quenched by applying both compression and extension forces and the quenching efficiencies differed in each case.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
