Abstract
Many cells demonstrate variances in behavior due to their cell culture environment. To provide a mechanically-tunable, tissue-like environment, a 3D hydrogel for cell culture was formulated using collagen (Col) and hyaluronic acid (HA) to help provide a system for studying these dynamic cellular responses in a soft-tissue environment. A design of experiments was organized to study the effects of collagen concentration, HA fragment size, and Col: HA mass ratio on the hydrogel mechanical properties. Mechanical characterization of the gels was conducted using rheology and found that collagen concentration, but not HA content, directly modulated the hydrogel storage and loss modulus. The pore size of the hydrogels was also evaluated and found to trend directly with collagen concentration.
Published Version
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