Abstract
Zinc is the second most abundant transition metal on the body. While the nature of zinc binding to DNA remains unclear it is known that zinc binding causes measureable structural changes. It is not established whether the divalent zinc ions are specifically bound to a particular site within DNA or diffusively bound. By combining CD (circular dichroism) spectroscopy measurements of DNA with ICP-OES (Inductively coupled plasma optical emission spectroscopy) measurements of ions binding to DNA, changes caused by the zinc binding can be monitored. CD spectroscopy measurements oversee changes in the DNA structure, while ICP-OES can quantify the amount of zinc ions bound to the phosphate groups of DNA. This will provide important insight on the effects of transition metal divalent ion binding on DNA structure.
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